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her2 positive bc cell line skbr3  (ATCC)


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    Structured Review

    ATCC her2 positive bc cell line skbr3
    Her2 Positive Bc Cell Line Skbr3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6543 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sk+br/SK-BR-3/10__1186_slash_s12906___026___05523___y-40-0-9
    Average 99 stars, based on 6543 article reviews
    her2 positive bc cell line skbr3 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    RNA sequencing:

    Article Title: Methods for in situ transcriptomics and proteomics
    Article Snippet: .. Based on RNAseq data reported on The Human Protein Atlas, the SK-BR-3 human breast cancer cell line (ATCC HTB-30TM) has high expression of the ERBB2 gene product, while the U-138MG glioblastoma cell line (ATCC HTB-16TM) has high expression of the MEIS2 gene product, relative to each other (see, e.g., Table 1; data for ERBB2 acquired from www.proteinatlas.org/ENSG00000141736-ERBB2/cell #rna, and data for MEIS2 acquired from www.proteinatlas.org/ENSG00000134138-MEIS2/cell #rna). ..

    Expressing:

    Article Title: Methods for in situ transcriptomics and proteomics
    Article Snippet: .. Based on RNAseq data reported on The Human Protein Atlas, the SK-BR-3 human breast cancer cell line (ATCC HTB-30TM) has high expression of the ERBB2 gene product, while the U-138MG glioblastoma cell line (ATCC HTB-16TM) has high expression of the MEIS2 gene product, relative to each other (see, e.g., Table 1; data for ERBB2 acquired from www.proteinatlas.org/ENSG00000141736-ERBB2/cell #rna, and data for MEIS2 acquired from www.proteinatlas.org/ENSG00000134138-MEIS2/cell #rna). ..

    other:

    Article Title: Antibody, linkers, payload, conjugates and applications thereof
    Article Snippet: MS: Thermo Fisher Q Exactive Plus, Water2795-Quattro micro triple quadrupole mass spectrometer HPLC: Waters 2695, Agilent 1100, Agilent 1200 Semi-preparative HPLC: Lisure HP plus 50D Flow Cytometry: CytoFLEX S HIC-HPLC: Butyl-HIC; mobile phase A: 25 mM PB, 2M (NH4)2SO4, pH 7.0; mobile phase B: 25 mM PB, pH 7.0; flow rate: 0.8 ml/min; acquisition time: 25 min; injection amount: 20 μg; column temperature: 25° C.; detection wavelength: 280 nm; sample chamber temperature: 8° C. SEC-HPLC: column: TSK-gel G3000 SWXL, TOSOH 7.8 mm ID×300 mm, 5 μm; mobile phase: 0.2 M KH2PO4, 0.25 M KCl, pH 6.2; flow rate: 0.5 ml/min; acquisition time: 30 min; injection volume: 50 μl; column temperature: 25° C.; detection wavelength; 280 nm; sample tray temperature: 8° C. CHO was obtained from Thermo Fisher Scientific; pcDNA 3.3 was obtained from Life Technology; HEK293F was obtained from Prejin; PEIMAX transfection reagent was obtained from Polyscience; MabSelect Sure ProA was obtained from GE; Capto S ImpAct was obtained from GE; Rink-amide-MBHA-resin and dichloro resin were obtained from Nankai synthesis; HCC1954 was obtained from ATCC CAT #CRL-2338; SK-BR-3 was obtained from ATCC CAT #HTB-30; BT-474 was obtained from ATCC CAT #HTB-20; NCI-N87 cells was obtained from ATCC CAT #CRL-5822; MCF7 was obtained from ATCC CAT #HTB-22; MDA-MB-231 was obtained from ATCC CAT #HTB-26; MDA-MB-468 was obtained from ATCC CAT #HTB-132; CFPAC-1 was obtained from ATCC CAT #CRL-1918; NCI-H2110 was obtained from ATCC CAT #CRL-5924; JIMT-1 was obtained from Wuxi Apptech; Capan-1 was obtained from ATCC CAT #CRL-1573; optimized recombinant enzyme Sortase A derived from Staphylococcus aureus is prepared in E. coli.

    Activity Assay:

    Article Title: De novo masking domains that gate TNF-family ligand assembly and activity
    Article Snippet: Data were analyzed in GraphPad Prism, and EC50 values were obtained by nonlinear regression using a four-parameter logistic model. .. Cytotoxic activity of ADCs was evaluated using SK-BR-3 human breast cancer cells (ATCC). ..

    Article Title: De novo masking domains that gate TNF-family ligand assembly and activity
    Article Snippet: .. Cell binding activity of antibodies and ADCs was evaluated using SK-BR-3 human breast cancer cells (ATCC). ..

    Cell Culture:

    Article Title: A novel synthetic melanin as a potential anticancer agent that induces apoptosis and cyclin D downregulation through distinct pathways.
    Article Snippet: .. 692 693 Cell line and cell culture 694 Human cancer cell lines, HeLa (uterine cervix), HepG2 (liver), Jurkat (T lymphocyte), SK-BR-3 695 (breast) cell lines were purchased from ATCC (Manassas, VA). ..

    Article Title: HSP90AA1-VDAC1 interaction contributes to breast cancer progression and doxorubicin resistance through PI3K/AKT signaling.
    Article Snippet: .. Cell culture 124 The normal mammary epithelial cell line MCF-10A and BC cell 125 lines MCF-7, BT474, MDA-MB-231, and SK-BR-3 were obtained 126 from the American Type Culture Collection (ATCC). ..

    Multiple Displacement Amplification:

    Article Title: HSP90AA1-VDAC1 interaction contributes to breast cancer progression and doxorubicin resistance through PI3K/AKT signaling.
    Article Snippet: .. Cell culture 124 The normal mammary epithelial cell line MCF-10A and BC cell 125 lines MCF-7, BT474, MDA-MB-231, and SK-BR-3 were obtained 126 from the American Type Culture Collection (ATCC). ..

    Article Title: HSP90AA1–VDAC1 interaction contributes to breast cancer progression and doxorubicin resistance through PI3K/AKT signaling
    Article Snippet: .. The normal mammary epithelial cell line MCF-10A and BC cell lines MCF-7, BT474, MDA-MB-231, and SK-BR-3 were obtained from the American Type Culture Collection (ATCC). ..

    Binding Assay:

    Article Title: De novo masking domains that gate TNF-family ligand assembly and activity
    Article Snippet: .. Cell binding activity of antibodies and ADCs was evaluated using SK-BR-3 human breast cancer cells (ATCC). ..



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